Anna Theresa Cavasin
Improving 3D reconstruction methods for single particle cryo-electron microscopy
Single particle cryo-electron microscopy has evolved into one of the standard techniques in structural biology in recent years. Purified molecules in aqueous solution are rapidly frozen and then analyzed by transmission electron microscopy. As a single particle technique, the method allows for the elucidation of large macromolecular complexes and for inferring information on protein dynamics. The aim of the project is to improve the state of the art computational methods for density map generation from experimental cryo-EM images, to pave the way for new developments in structure-based drug design.